SIRTE (BNO NEWS) — A video released by the Global Post appears to capture an unidentified man sodomizing ousted Libyan leader Colonel Muammar Gaddafi with an object before he was eventually killed.

The video was released on Friday but a closer review of the footage on Saturday appears to show a person bending over and inserting a thin piece of wood inside Gaddafi’s bottom. The video was released by the website Global Post, which obtained the footage from rebel fighters.

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Gaddafi was captured on Thursday morning by fighters supporting Libya’s transitional government as he tried to flee his hometown Sirte. He was eventually shot dead, but it remains unclear whether he was executed by his captors or if he was killed during fighting between pro-Gaddafi and anti-Gaddafi forces.

On Friday, Amnesty International urged the new Libyan authorities to ensure a full, independent and impartial investigation into how Gaddafi died. “If Colonel Gaddafi was killed after his capture, it would constitute a war crime and those responsible should be brought to justice,” said Claudio Cordone, Senior Director at Amnesty International.

Cordone added: “Investigating whether or not his death was a war crime might be unpopular. However, the NTC (National Transitional Council) must apply the same standards to all, affording justice even to those who categorically denied it to others.”

A spokesman for the United Nations human rights office also called for an investigation on Friday, describing images of Gaddafi’s last moments as ‘very disturbing’. Libyan authorities previously said Gaddafi, who ruled the African country from 1969 until earlier this year, died of a gunshot wound to the head.

Libya has been engulfed in a civil war since an uprising against Muammar Gaddafi’s regime began in mid-February, while pro-Gaddafi forces have been accused of violently cracking down on anti-government protesters. It led to UN Security Council Resolution 1973 which authorized member states to impose a no-fly zone over the North African country and to take ‘all necessary measures’ to protect civilians.

It is believed more than 25,000 people have been killed during the civil war.